论文部分内容阅读
对金针菇Flammulina velutipes单核菌丝W23的菌丝体以及与L11质配后的双核菌丝H1123菌丝体进行了转录组测序,以本实验室已获得的W23基因组为参考基因组研究两样本间差异基因,并对这些差异基因进行了GO功能和Pathway显著性富集分析。差异基因分析显示,两个样本中共有显著性差异表达的基因3 504个,其中在双核菌丝中上调、下调的基因数分别为2 151和1 353个。研究发现差异表达基因含有很多的转录因子基因、蛋白激酶以及WD40 repeat-like蛋白。Gene Ontology(GO)功能分析结果表明,extracellular region和membrane-enclosed lumen条目下的差异基因全部为上调表达,而envelope下的差异基因全部为下调表达,以利于双核菌丝分裂时锁状联合的形成而便于核的迁移。Pathway功能富集分析结果表明,脂肪酸、氨基酸以及大部分糖类合成相关基因具有比较活跃的上调表达。说明双核菌丝主要进行营养物质的富集,为下一步在合适条件下分化成原基,进入生殖生长阶段储备物质基础。
The mycelia of Flammulina velutipes mononuclear W23 mycelia and the L11 hyphal hyphae of H1123 mycelia were transcribed and sequenced. The W23 genome obtained in this laboratory was used as a reference genome to study the differences between the two samples Genes, GO gene and Pathway significant enrichment analysis of these differentially expressed genes. Differential gene analysis showed that there were 3 504 differentially expressed genes in both samples, of which 2 151 and 1 353 genes were up-regulated and down-regulated respectively in the double-hyphae. The study found that differentially expressed genes contain many transcription factor genes, protein kinases and WD40 repeat-like proteins. Gene Ontology (GO) functional analysis showed that the differential genes under the extracellular region and membrane-enclosed lumen entries were all up-regulated, while the genes under envelope were all down-regulated to facilitate the formation of the lock-like association during binucleate myelodysplasia And easy to nuclear migration. Pathway enrichment analysis showed that fatty acids, amino acids and most of the genes related to carbohydrate synthesis have more active up-regulated expression. It shows that the main fungi of dual-core mycelium enrichment, the next step under the appropriate conditions to differentiate into the original base, enter the reproductive growth stage of the material basis for the reserve.