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利用地高辛配基标记的HBV DNA及HDVcDNA探针,原位杂交检测了一例胆管细胞性肝癌及癌旁肝组织中的HBV DNA和HDV RNA。在癌巢及癌旁肝组织中均检出HBV DNA及HDV RNA阳性细胞,以核型为主。癌巢中阳性细胞数及阳性信号强度明显高于癌旁肝组织。经阴性对照、空白对照及DNase I,RNase A处理试验,证实阳性信号为HBV DNA或HDV RNA产生。进一步用逆转录一聚合酶链反应(RT-PCR)检出了HDV RNA,确证了HDV感染的存在。
Digoxigenin-labeled HBV DNA and HDV cDNA probes were used to detect HBV DNA and HDV RNA in one case of cholangiocarcinoma and adjacent liver tissues using in situ hybridization. HBV DNA and HDV RNA-positive cells were detected in cancer nests and adjacent liver tissue, with karyotypes as the main components. The number of positive cells and the positive signal intensity in the cancer nest were significantly higher than those in the adjacent liver tissue. By negative control, blank control, and DNase I, RNase A treatment test, it was confirmed that the positive signal was HBV DNA or HDV RNA production. Further detection of HDV RNA by reverse transcription-polymerase chain reaction (RT-PCR) confirmed the presence of HDV infection.