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目的:建立理气复胃口服液中木香烃内酯和去氢木香内酯的含量测定的HPLC方法。方法:色谱柱为Discovery C18 (150 mm×4．6 mm,5μm),柱温30℃,流动相为甲醇-乙腈-水(60:5:35),流速1．0 ml·min-1,检测波长225nm。结果:木香烃内酯和去氢木香内酯的进样量在0．2-2．0μg范围内,与其峰面积有良好的线性关系;平均加样回收率分别为97．4％, 97．9％,RSD分别为1．5％,1．3％。结论:方法简便易行、准确可靠,可用于控制该产品的质量。
OBJECTIVE: To establish a HPLC method for the determination of costunolide and dehydrocostuslactone in Liqi Fuwei Oral Liquid. Method: The chromatographic column was a Discovery C18 (150 mm×4.6 mm, 5 μm) column temperature of 30°C. The mobile phase was methanol-acetonitrile-water (60:5:35) with a flow rate of 1.0 ml·min-1. Detection wavelength 225nm. RESULTS: The injection amount of the woody lactone and dehydrocostus lactone was in the range of 0.2-2.0 μg, and there was a good linear relationship with the peak area. The average recovery rate was 97.4%. 97.9%, RSD were 1.5%, 1.3%. Conclusion: The method is simple, accurate, and reliable, and can be used to control the quality of the product.