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目的:探讨低频率低强度脉冲电磁场(PEMFs)对绝经后骨质疏松症大鼠的影响。方法:选取3-5月龄雌性SD大鼠,随机分为五组,即阴性对照组(Sham)、模型组(Model)、阳性对照组(XLGB)、脉冲磁场照射组(PEMFs)和给药+照射治疗组(X+P),除Sham实行假性手术外,其余四组经手术分别摘取两侧卵巢(OVX)。OVX术后1月按照分组开始治疗,3个月后处死动物,测量大鼠骨转化生化指标(血清ALP、OC和尿DPD),股骨总BMD、BMC,股骨干骺端骨小梁微结构(BV/TV、Conn.D、SMI、Tb·N、Tb·Th、Tb·Sp及Tb·Ar)、骨生物力学性能(Stress)变化并计算。结果:XLGB、PEMFs、X+P组BMD、BMC、Tb·N、Tb·Th值显著增强, SMI、Tb·Sp值显著减小(P<0.01);ALP值有所减小,BV/TV、Conn.D值有所增加(P<0.05);Sham组Stress值有明显差异(P<0. 01),PEMFs组有一定差异(P<0.05);提示经过磁场照射和(或)药物治疗后股骨生物力学显著增强,骨组织结构骨质疏松症状得到明显改善。结论:PEMFs对绝经后雌激素缺乏引起的OP大鼠有较好的治疗作用。
Objective: To investigate the effects of low-frequency low-intensity pulsed electromagnetic fields (PEMFs) on postmenopausal osteoporosis rats. Methods: Female Sprague-Dawley rats aged 3-5 months were randomly divided into five groups: Sham, Model, XLGB, PEMFs, + Irradiation treatment group (X + P). Except Sham undergoing pseudo-surgery, the other four groups were surgically removed ovaries on both sides (OVX). The animals were sacrificed 3 months after OVX treatment. The biochemical markers of bone turnover (serum ALP, OC and urinary DPD), total femur BMD, BMC, and trabecular bone microstructure of femoral metaphysis BV / TV, Conn.D, SMI, Tb · N, Tb · Th, Tb · Sp and Tb · Ar) were measured and the changes of biomechanical properties were calculated and calculated. Results: The values of BMD, BMC, Tb · N, Tb · Th in XLGB, PEMFs and X + P groups were significantly increased (P <0.01) (P <0.05). There was a significant difference in stress values between Sham group (P <0.01) and PEMFs group (P <0.05). It suggested that after magnetic field irradiation and / or drug treatment After femoral biomechanics significantly enhanced, osteoporotic symptoms of bone tissue has been significantly improved. Conclusion: PEMFs have a good therapeutic effect on OP rats induced by estrogen deficiency after menopause.