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叶绿素相关突变体是水稻功能基因组学研究的一类重要突变源,对于揭示水稻叶绿素生物合成机制以及构成光合系统蛋白复合体的相关研究都具有重要意义.利用EMS诱变籼稻恢复系缙恢10号,得到一个新的全生育期黄绿叶突变体ygl4(t),表型分析结果显示,该突变体的剑叶长、有效穗与每穗粒数与野生型(WT)差异无统计学意义,而株高、千粒质量、结实率等7个性状均显著或极显著低于WT.此外,叶绿素a(Chla)质量分数,净光合速率(Pn),蒸腾速率(Tr)与WT差异无统计学意义,而叶绿素b(Chlb)质量分数,总叶绿素(Chl)质量分数,Chla/Chlb的比值,气孔导度(Gs)与胞间CO2浓度(Ci)显著或极显著低于WT.突变体叶绿体内基粒片层减少,排列不规则,致使叶绿体在发育进程中受到抑制.遗传分析表明,该性状受1对隐性核基因控制,YGL4(t)被位于水稻第10染色体RM1162与RM7093之间,物理距离约400kb.序列分析表明,编码叶绿素a氧化酶(chlorophyll a oxygenase)基因OsCAO1在终止密码子下游第12个碱基由G置换成T,推测OsCAO1即为YGL4(t)的候选基因.该研究为YGL4(t)基因功能研究奠定了基础.
Chlorophyll-related mutants are important mutational sources in rice functional genomics research and are of great significance for revealing the mechanism of rice chlorophyll biosynthesis and the related research of photosynthetic system protein complex.Using EMS to mutagenize Indica restorer line Jinhui 10 , A new yellow-green leaf mutant, ygl4 (t), was obtained. Phenotypic analysis showed that there was no significant difference in the flag leaf length, effective panicle, grain number per panicle and wild type (WT) However, seven traits such as plant height, 1000-grain weight and seed setting rate were all significantly or extremely significantly lower than those of WT. In addition, no significant differences were found in the contents of chlorophyll a (Chla), net photosynthetic rate (Pn), transpiration rate (Tr) The chlorophyll b (Chlb), total chlorophyll (Chl), Chla / Chlb ratio, stomatal conductance (Gs) and intercellular CO2 concentration (Ci) were significantly or extremely significantly lower than those of WT The results showed that the trait of chloroplast was controlled by one pair of recessive nuclear genes, and YGL4 (t) was located at RM1162 and RM7093 on chromosome 10 of rice Between the physical distance of about 400kb. Sequence analysis table , The gene encoding the chlorophyll a oxygenase (OsCAO1) at the 12th nucleotide downstream of the stop codon was replaced by G, suggesting that OsCAO1 is a candidate gene of YGL4 (t) Research laid the foundation.