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目的利用质谱技术对类风湿关节炎(RA)患者关节滑膜中的免疫复合物进行全面的抗原谱分析。方法选取本院3例RA患者和3例骨关节炎(osteoarthritis,OA)患者的关节滑膜,通过蛋白质提取、免疫沉淀、胶内酶解和液相色谱串联质谱系统,对滑膜免疫复合物进行抗原谱分析。用FOT值(fraction of total)评价蛋白质的丰度,通过FOT值的比较筛选出上调蛋白和下调蛋白。用David和String软件对差异蛋白质进行功能富集、相互作用网络和信号通路分析。结果在RA和OA患者的关节滑膜组织免疫复合物中分别鉴定出511和526种蛋白质。通过维恩图比较,仅在RA组出现的蛋白质有170种。RA组有45种蛋白质发生上调,85种蛋白质发生下调。结论热休克蛋白HSP90AA1、HSP70、人类白细胞抗原(HLA)-G、硫氧还蛋白、膜联蛋白A2和玻连蛋白可能通过不同的机制参与RA的发病过程,具有RA新的生物标志物的潜在应用价值。
OBJECTIVE: To perform a comprehensive antigen-based spectral analysis of immune complexes in the synovium of patients with rheumatoid arthritis (RA) using mass spectrometry. Methods Synovial synovium of 3 patients with RA and 3 patients with osteoarthritis was selected and compared with each other by protein extraction, immunoprecipitation, gel electrophoresis and tandem mass spectrometry (LC-MS / MS) Antigen profiling was performed. Protein abundance was assessed by fraction of total, and up-regulated and down-regulated proteins were screened by FOT values. Differentially proteins were enriched by David and String software and analyzed by interaction networks and signal pathways. Results 511 and 526 proteins were identified in synovial tissue immune complexes of RA and OA patients, respectively. Via Venn diagram, only 170 proteins appeared in RA group. In RA group, 45 proteins were up-regulated and 85 proteins were down-regulated. Conclusions The heat shock proteins HSP90AA1, HSP70, HLA-G, thioredoxin, annexin A2 and vitronectin may participate in the pathogenesis of RA through different mechanisms and have the potential of new RA biomarkers Value.