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目的:观察活血化瘀方剂中不同剂量黄芪组方对抗氧化作用的影响,以探讨黄芪适宜的使用剂量。方法:分别采用牛的肾匀浆自发性脂质过氧化,H2O2诱导肾匀浆脂质过氧化和诱导红细胞溶血的生物测定法。结果:模型组MDA含量明显升高。与空白对照组比较有显著性差异(P<0.01);A药组、B药组、C药组、维生素E组能明显降低MDA含量,与模型组比较均有显著性差异(P<0.01);A、C药组降低MDA含量作用弱于B药组。中药组方体外能显著抑制H2O2诱导的红细胞氧化溶血。A药组、B药组、C药组、维生素E组能明显抑制溶血,与模型组比较均有显著性差异,A、C药组作用弱于B药组。结论:在治疗缺血性脑血管疾病的益气活血复方配伍中,黄芪的用量宜大。但随着其剂量的增大,其抗氧化效应反而减弱。
OBJECTIVE: To observe the anti-oxidation effects of different doses of Radix Astragali in the prescription of Huoxue Huayu Prescription, so as to discuss the appropriate dose of Radix Astragali. METHODS: The lipid peroxidation of renal homogenate and the hemolysis of red blood cells induced by H2O2 were measured by the spontaneous lipid peroxidation of bovine kidney homogenate. Results: The MDA content in the model group increased significantly. Compared with the control group, there was a significant difference (P<0.01); A drug group, B drug group, C drug group, vitamin E group can significantly reduce the MDA content, compared with the model group there was a significant difference (P<0.01) ; A, C drug group to reduce MDA content is weaker than B drug group. The Chinese medicine prescription can significantly inhibit H2O2-induced red blood cell oxidative hemolysis in vitro. A drug group, B drug group, C drug group, vitamin E group can significantly inhibit hemolysis, compared with the model group had significant differences, A, C drug group was weaker than B drug group. Conclusion: In the combination therapy of Yiqi Huoxue for the treatment of ischemic cerebrovascular diseases, the dosage of Huangqi should be large. However, as its dose increases, its anti-oxidant effect weakens.