论文部分内容阅读
目的:研究绿茶浸出物与头孢唑啉合用对耐甲氧西林金黄色葡萄球菌(MRSA)的抗菌机制。方法:高效液相色谱法测定头孢唑啉与绿茶及其提取物合用和头孢唑啉单用处理的MRSA和ATCC25923菌体内头孢唑啉的含量。应用不同浓度的绿茶浸出物或绿茶浸出物-氯化钠溶液检测MRSA在低渗和高渗环境中的耐受能力。结果:经过绿茶浸出物、茶多酚、表儿茶素(EC)、表儿茶素没食子酸酯(ECg)和表没食子儿茶素没食子酸酯(EGCg)与头孢唑啉合用处理的MRSA和ATCC25923组菌体内的头孢唑啉的含量高于单用头孢唑啉组。随着绿茶浸出物和氯化钠浓度的上升,MRSA菌的耐受能力降低。结论:绿茶及其提取物促进头孢唑啉在细菌体内蓄积。经绿茶处理后的MRSA的细胞壁可能受损。
Objective: To study the antibacterial mechanism of green tea extract combined with cefazolin on methicillin-resistant Staphylococcus aureus (MRSA). Methods: The content of cefazolin in MRSA and ATCC25923 cells treated with cefazolin, green tea and their extracts and cefazolin alone were determined by high performance liquid chromatography. Different concentrations of green tea extract or green tea extract - sodium chloride solution were used to test MRSA tolerance in the hypotonic and hypertonic environments. Results: MRSA and cefazolin treated with green tea extract, tea polyphenol, epicatechin (EC), epicatechin gallate (ECg) and epigallocatechin gallate (EGCg) The content of cefazolin in the ATCC25923 group was higher than that in the single cefazolin group. As the concentration of green tea extract and sodium chloride increases, the tolerance of MRSA bacteria decreases. Conclusion: Green tea and its extract can promote the accumulation of cefazolin in bacteria. The cell wall of MRSA after green tea treatment may be damaged.