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成熟大弹涂鱼的卵巢、精巢和储精囊稀释液中的性外激素以及缩宫素和脱氧皮质酮 (DOC)能诱导第 4时相的大弹涂鱼卵母细胞在离体培养的条件下发育成熟 ,成熟率与性外激素的种类和剂量有关 .前列腺素E1(PGE1)单独使用的效果不明显 ,但与人绒毛膜促性腺激素 (HCG)混合使用时显著提高了卵母细胞的成熟率 .以卵巢、精巢和储精囊稀释液以及PGE1分别注射雌性大弹涂鱼 ,剂量为 0 .0 1cm3/尾的卵巢液、0 .1cm3/尾的储精囊液、0 .1cm3/尾的精巢液以及剂量为 1 0 μg/尾的PGE1都有诱导卵母细胞成熟的作用 ,其中储精囊液的作用最为显著 .在人工管道中放置大弹涂鱼成熟亲鱼卵巢、精巢或储精囊的稀释液 ,有诱导异性亲鱼进入管道内的作用 ,其中以储精囊液对雌鱼的吸引作用尤为明显 .放置于陶瓷管道或海绵管道内的卵巢、精巢或储精囊稀释液都能诱导大弹涂鱼产卵 ,在陶瓷管道的产卵量和受精率显著高于海绵管道 .
Ovaries, testis and seminal vesicle diluents in ovaries, testes, and oxytocin (DOC) -induced the development of matured oocytes in the fourth phase, The maturation rate is related to the type and dose of sex pheromone.The effect of prostaglandin E1 (PGE1) alone is not obvious, but when mixed with human chorionic gonadotropin (HCG), the maturation rate of oocytes is significantly increased Ovary, testis and seminal vesicle diluent and PGE1 were injected female large-billed fish, the dose of 0 .0 1cm3 / tail ovarian liquid, 0 .1cm3 / tail of the seminal vesicle fluid, 0 .1cm3 / tail of the testis and the dose of 1 0 μg / tail of PGE1 induced oocyte maturation, of which the most important role of seminal vesicle fluid in the artificial tube placed in a large tube of mature fish broodstock ovary, sperm or seminal vesicle dilution, inducing heterosexual brooder into the pipeline Especially the seminal vesicle fluid on females.The ovary, testis or seminal vesicle diluent placed in the ceramic pipe or sponge pipe can induce the spawning of the big billed fish, Pipeline spawning and fertilization rates were significantly higher than sponge pipelines.