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目的:观察选择性COX-2抑制剂塞来昔布(Celecoxib)诱导结肠腺癌细胞株Ls174凋亡的作用及机制。方法:采用MTT法检测塞来昔布对Ls174细胞的增殖抑制作用,应用流式细胞仪检测塞来昔布对Ls174细胞凋亡和增殖周期的影响,并检测塞来昔布对Ls174细胞Caspase-3活性的影响。结果:塞来昔布抑制Ls174细胞增殖和诱导Ls174细胞的凋亡作用均呈剂量和时间依赖性,塞来昔布处理后的Ls174细胞G0/G1期百分比与对照组相比明显增高。塞来昔布能诱导Ls174细胞Caspase-3活性,并呈浓度和时间依赖性。结论:塞来昔布在体外能抑制Ls174细胞的增殖,诱导细胞凋亡,其作用与促进Caspase-3活性和阻滞细胞周期于G0/G1期有关。
Objective: To observe the effect and mechanism of selective COX-2 inhibitor Celecoxib on apoptosis of colon adenocarcinoma cell line Ls174. Methods: MTT assay was used to detect the inhibitory effect of celecoxib on the proliferation of Ls174 cells. The effect of celecoxib on apoptosis and proliferation of Ls174 cells was detected by flow cytometry. The effect of celecoxib on the expression of Caspase- 3 activity. RESULTS: Celecoxib inhibited the proliferation of Ls174 cells and induced the apoptosis of Ls174 cells in a dose-and time-dependent manner. The percentage of G0 / G1 phase of Ls174 cells treated with celecoxib was significantly higher than that of the control. Celecoxib induced Caspase-3 activity in Ls174 cells in a concentration- and time-dependent manner. CONCLUSION: Celecoxib can inhibit the proliferation of Ls174 cells in vitro and induce apoptosis. The effect of Celecoxib on promoting the activity of Caspase-3 and arresting the cell cycle at G0 / G1 phase.