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本文介绍了10株互隔交链孢霉提取物诱发人淋巴细胞姐妹染色单体互换(SCE)及染色体畸变。结果发现,当用不同浓度提取物加入培养基时,有8株所诱发的SCE增高,与对照组比较差异极为显著(P<0.01)、在8株阳性株中,有6株在加入低量提取物时即诱发SCE频率增高显示统计学差异、同时,在阳性株中有两株提取物所诱发的染色体畸变频率增高,与对照组比较亦有极显著性差异(P<0.01)。由此可见,多数互隔交链孢霉菌株(8/10)所产生的代谢产物或毒素,都含有致突变物,不同菌株具有不同强度的致突作用。另外,对应用人淋巴细胞的SCE技术检测致突变物的优点进行了讨论。
This article describes the induction of human sister cell chromatid exchange (SCE) and chromosomal aberrations in human lymphocytes induced by 10 isolates of A. alternifolia. As a result, it was found that when the different concentrations of the extract were added to the culture medium, 8 SCEs induced by the extract were significantly increased (P<0.01), and among the 8 positive strains, 6 were added at a low level. The frequency of SCE induced by extracts showed a statistically significant difference. At the same time, the frequency of chromosomal aberrations induced by two extracts in positive strains increased, and there was a significant difference compared with the control group (P<0.01). It can be seen that most of the metabolites or toxins produced by Alternaria alternata strains (8/10) contain mutagens, and different strains have different intensity of the convulsions. In addition, the advantages of detecting mutagen using SCE technology of human lymphocytes were discussed.