1951年Krakower和Greenspon首先分离了肾小球基膜(Glomerular basement membrane,简称GBM),为了提高GBM的纯度和产量不妨加以改变或改进。在肾病综合征免疫的研究中,我们制备了可溶性胎儿GBM抗原,制备原则仍按Westberg等法,略有改变。纯度鉴定则按Misra等法。 用胎龄4～8个月的48个胎儿的肾脏制备肾小球。把过滤的筛网自200目改为250目(孔径74μ)。 1951 Krakower and Greenspon first isolated glomerular basement membrane (GBM), in order to improve the purity and yield GBM may wish to change or improve. In the study of nephrotic syndrome immunization, we prepared soluble fetal GBM antigen, the principle of preparation is still a slight change according to Westberg law. Purity identification according Misra method. Glomeruli were prepared from the kidneys of 48 fetuses of 4-8 months gestational age. The sieve was changed from 200 mesh to 250 mesh (pore size 74μ).