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目的 构建以组蛋白为核心的新型靶向性基因导入系统。方法 将各功能肽与核心区成分设计成融合基因 ,在大肠杆菌中加以表达 ,获得的融合蛋白通过静电效应与DNA结合形成靶向性的基因导入系统 ,通过体内外报告基因导入试验检测生物学活性。结果 经体内外基因导入实验证明 ,制备的融合蛋白能将外源报告基因靶向性地导入肿瘤细胞和组织。结论 运用基因工程方法制备的融合蛋白适合大规模生产 ,便于质控 ,为靶向性基因导入系统应用于基因治疗提供了新的思路。
Objective To construct a novel target gene delivery system with histone as the core. Methods The functional peptide and core region were designed as fusion genes and expressed in E. coli. The obtained fusion protein was combined with DNA by electrostatic effect to form targeted gene introduction system. The in vivo and in vitro reporter gene test was used to test the biology active. Results In vitro and in vivo gene transfer experiments demonstrated that the prepared fusion protein can target foreign gene into tumor cells and tissues. Conclusion The fusion protein prepared by genetic engineering method is suitable for mass production and is convenient for quality control. It provides a new idea for targeted gene introduction system for gene therapy.