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【目的】研究盐胁迫下印度梨形孢定殖对豆科模式植物蒺藜苜蓿生长发育的影响。【方法】通过分析不同生境下植物的根长、根鲜重和茎鲜重,以及体内抗氧化物酶活性、脯氨酸含量、甜菜碱醛脱氢酶基因(BADH)的表达,确定印度梨形孢对蒺藜苜蓿生长的促进作用,并初步阐释印度梨形孢诱导植物耐盐性的机制。【结果】印度梨形孢能在蒺藜苜蓿根部定殖并能促进植物的生长发育,有效缓解盐胁迫造成的生长抑制。印度梨形孢能提高植物体内抗氧化物酶活性,增加游离脯氨酸含量并诱导BADH基因的表达。【结论】印度梨形孢作为植物生长促进因子可以用来提高植物耐盐性,实现盐碱土壤的间接改良。
【Objective】 The objective of this study was to investigate the effects of Pythium sp. Colonization on the growth and development of Medicago truncatula under salt stress. 【Method】 The root length, root fresh weight and stem fresh weight of plants under different habitats, and the antioxidant enzyme activity, proline content and expression of betaine aldehyde dehydrogenase gene (BADH) The promotion of Medicago truncatula to the growth of Medicago truncatula and preliminary elucidation of the mechanism of salt tolerance induced by the Indian pear spore. 【Result】 The result showed that P. pyriformia could colonize the roots of Medicago truncatula and promote the growth and development of plants, and effectively alleviate the growth inhibition caused by salt stress. Indian pear spores can improve plant antioxidant enzyme activity, increase free proline content and induce BADH gene expression. 【Conclusion】 Indian pear spore as a plant growth promoting factor can be used to improve plant salt tolerance, to achieve indirect improvement of saline-alkali soil.