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采用改良的分光光度法及豌豆凝集素亲和免疫电泳,初步研究了肝细胞癌(HCC)患者血清α—L一岩藻糖苷酶(AFU)对甲胎蛋白(AFP)糖基上岩藻糖的水解活性,旨在探讨HCC酶标记物与蛋白质标记物在生化代谢过程中的相互关系。结果表明:(1)当牛AFU(美国Sigma)以0.3U/ml含量与岩藻糖化AFP纯品同溶于pH4.5,0.025mol/L柠檬酸钠缓冲液,在37℃温育24h后发现,人AFP分子上的岩藻糖基可
A modified spectrophotometric method and pea-lectin affinity immunoelectrophoresis was used to preliminarily study the serum α-L fucosidase (AFU) and α-fetoprotein (AFP) fucose-based fucose in patients with hepatocellular carcinoma (HCC). The hydrolytic activity aims to explore the relationship between HCC enzyme markers and protein markers in biochemical metabolism. The results showed that: (1) When cattle AFU (Sigma, USA) was dissolved in pH 4.5 and 0.025 mol/L sodium citrate buffer at a concentration of 0.3 U/ml with fucosylated AFP, incubated at 37°C for 24 hours It was found that fucosyl groups on human AFP molecules can be