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[Objective] To establish a method for simultaneous determination of resveratrol,polydatin,emodin and physcion in Giant Knotweed Rhizome by HPLC.[Methods] The HPLC method was established with DIKMA C18 column(200 mm×4.6 mm,5 μm)and a mobile phase of acetonitrile-0.1% phosphoric acid.The flow rate was 1.0 ml/min;the detection wavelength was 303 nm and the column temperature was 30 ℃.[Results]the linear regression equation of resveratrol,polydatin,emodin and physcion were y = 7 116 169x + 15 631(r=0.999 9),y= 395 313x + 50(r=0.999 9),y=2 752 869x-266 4(r=0.999 9) and y = 463 866x-1 413(r=0.999 9),and present good linear relationship within 0.026-0.520 μg,0.045-0.765 μg,0.026-0.520 μg and 0.026-0.442 μg,respectively.[Conclusion]The method is feasible with good repeatability,and can be used for quality control of Giant Knotweed Rhizome.
[Objective] To establish a method for simultaneous determination of resveratrol, polydatin, emodin and physcion in Giant Knotweed Rhizome by HPLC. [Methods] The HPLC method was established with DIKMA C18 column (200 mm × 4.6 mm, 5 μm) and a mobile phase of acetonitrile-0.1% phosphoric acid. The flow rate was 1.0 ml / min; the detection wavelength was 303 nm and the column temperature was 30 ° C. [Results] the linear regression equation of resveratrol, polydatin, emodin and physcion were y = 7 116 169x + 15 631 (r = 0.999 9), y = 395 313x + 50 (r = 0.999 9), y = 2 752 869x- 266 4 (r = 0.999 9) and y = 463 866x- 1 413 = 0.999 9), and present good linear relationship within 0.026-0.520 μg, 0.045-0.765 μg, 0.026-0.520 μg and 0.026-0.442 μg, respectively. [Conclusion] The method is feasible with good repeatability, and can be used for quality control of Giant Knotweed Rhizome.