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目的 在体外高糖刺激人肾脏间质成纤维细胞时观察其肝细胞生长因子 (HGF)和c met的表达。方法 将人肾脏间质成纤维细胞分别培养于不同浓度的葡萄糖中 ,于 2 4h后用RT PCR方法检测HGF、c met和 1型纤溶酶原激活物抑制物 (PAI 1)mRNA的转录水平 ,用不同浓度的HGF刺激细胞来观察PAI 1mRNA的表达。结果 高浓度葡萄糖培养的细胞早期就有HGF、c met表达 ,之后表达逐渐下降 ,而PAI 1在此之后出现表达上升。同时外源性HGF可以抑制PAI 1mRNA的表达。结论 HGF在肾脏中是潜在的一种抗纤维化的细胞因子 ,可以通过降低PAI 1的表达 ,从而促进细胞外基质的降解。
Objective To observe the expression of hepatocyte growth factor (HGF) and c-met in human renal interstitial fibroblasts stimulated by high glucose in vitro. Methods Human renal interstitial fibroblasts were cultured in different concentrations of glucose, and the transcriptional levels of HGF, c-met and PAI-1 mRNA were detected by RT-PCR after 24 hours The cells were stimulated with different concentrations of HGF to observe PAI 1 mRNA expression. Results The cells cultured in high concentration of glucose had HGF and c-met in the early stage, then decreased gradually, while the PAI 1 increased thereafter. At the same time exogenous HGF can inhibit PAI 1 mRNA expression. Conclusions HGF is a potential anti-fibrotic cytokine in kidneys, which can promote the degradation of extracellular matrix by decreasing PAI 1 expression.