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生长在氮素含量分别为0、75、150、300和600mg/钵下的盆栽大豆,其植株叶片的叶绿素含量与植株干重(r=0.93,P<0.01)、植株全氮量(r=0.87,P<0.01)显著相关。在与之平行的试验中,当共生有效性不同的快生型大豆根瘤菌分别接种于栽培大豆后,植株叶片的叶绿素含量与植株干重(r=0.98,P<0.01)、植株全氮量(r=0.89,P<0.01)和根瘤的固氮酶活性(r=0.95,P<0.01)也存在显著的相关性。利用叶绿素含量对供试快生型大豆根瘤菌共生有效性进行评估的结果与用植株全氮量的结果相似。研究证明可以用叶绿素含量来间接指示大豆植株的含氮量及根瘤菌的共生有效性。
Growth of potted soybean grown at 0, 75, 150, 300 and 600 mg / pot, respectively, showed a significant increase in leaf chlorophyll content and plant dry weight (r = 0.93, P <0.01) (R = 0.87, P <0.01) were significantly correlated. In a parallel experiment, chlorophyll content and plant dry weight (r = 0.98, P <0.01) of plant leaves after inoculation of quick-acting soybean rhizobia with different symbiosis availability were observed. There was also a significant correlation between plant total nitrogen (r = 0.89, P <0.01) and nodule nitrogenase activity (r = 0.95, P <0.01). Chlorophyll content was used to assess the symbiosis effectiveness of the fast-growing soybean rhizobia similar to that of the total nitrogen content of the plant. Studies have shown that chlorophyll content can be used to indirectly indicate the nitrogen content of soybean plants and rhizobia symbiosis effectiveness.