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目的：观察两种维甲酸（ ＲＡ） 对人胰腺癌细胞生长的抑制作用及其可能机制。方法：应用ＭＴＴ 比色法、流式细胞仪、裸鼠肾包膜下移植瘤抑制试验和透射电镜技术进行检测。结果：ＲＡ 抑制胰腺癌细胞生长，６ ｄ 组抑制５０ ％细胞生长的药物浓度（ＩＣ５０） 全反式ＲＡ（ＡＴＲＡ） 和１３ － 顺式（ＣＲＡ） 分别为１０ ～３０ μｍｏｌＬ 和３０ ～５０ μｍｏｌＬ。癌细胞在ＲＡ作用下，Ｇ０／Ｇ１ 期细胞比例增加，Ｓ 期细胞比例和增殖指数（ＰＩ） 下降。ＡＴＲＡ ６ ｄ 组凋亡指数（ ＡＩ） 增高，ＡＴＲＡ 和ＣＲＡ ６ ｄ组ＡＩＰＩ 比例分别是对照组的４ 倍和２ 倍。ＡＴＲＡ 能显著抑制肾包膜下胰腺癌移植瘤生长，电镜检查证实部分胰腺癌细胞具有凋亡特征。结论：ＲＡ 抗胰腺癌细胞生长作用主要与抑制细胞增殖有关
Objective: To observe the inhibitory effect of two retinoic acid (RA) on the growth of human pancreatic cancer cells and its possible mechanism. Methods: MTT colorimetric assay, flow cytometry, subcutaneous xenograft in nude mice and transmission electron microscopy were used for detection. Results: RA inhibited the growth of pancreatic cancer cells and the IC50 of 6 - day IC50 was 10 ~ 30 μmol·L -1 and 30 ~ 50 nm for ATRA and 13 - cis (CRA), respectively μmolL. Under the action of RA, the proportion of cells in G0 / G1 phase increased and the percentage of S phase cells and proliferation index (PI) decreased. ATRA 6 d group apoptosis index (AI) increased, ATRA and CRA 6 d AI PI ratio were 4 times and 2 times the control group. ATRA can significantly inhibit the growth of subrenal pancreatic cancer xenografts, electron microscopy confirmed that some pancreatic cancer cells have apoptotic features. Conclusion: The anti-pancreatic cancer cell growth of RA is mainly related to the inhibition of cell proliferation