Using the mesoscale eddy trajectory atlas product derived from satellite altimeter data from 1993 to 2016, this study analyzes statistical characteristics and seasonal variability of mesoscale eddies in the Banda Sea of the Indonesian seas. The results show that there were 147 mesoscale eddies that occurred in the Banda Sea, of which 137 eddies were locally generated and 10 originated from outside. The total numbers of cyclonic eddies (CEs, clockwise) and anticyclonic eddies (AEs, anticlockwise) are 76 and 71, respectively. Seasonally, the number of CEs (AEs) is twice larger than the number of AEs (CEs) in winter (summer). In winter, CEs are distributed in the southern and AEs in the northern basins, respectively, but the opposite thing occurs in summer, i.e., the polarities of mesoscale eddies observed at the same location reverse seasonally. The mechanisms of polarity distribution reversal (PDR) of mesoscale eddies are examined with reanalysis data of ocean currents and winds. The results indicate that the basin-scale vorticity, wind stress curl, and the meridional shear of zonal current reverse seasonally, which are favorable to the PDR of mesoscale eddies. The possible generation mechanisms of mesoscale eddies include direct wind forcing, barotropic and baroclinic instabilities, of which the direct wind forcing should play the dominant role.