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目的:对广西产柿叶进行质量分析研究,为进一步完善柿叶的质量标准,提供柿叶定性、定量的科学依据。方法:采用薄层色谱法对广西产柿叶进行定性鉴别;采用高效液相色谱法测定柿叶中齐墩果酸和熊果酸的含量,色谱柱为Thermo C18(4.6 mm×250 mm5,μm),流动相为甲醇-0.2%醋酸铵溶液(80∶20),流速0.8 mL/min,检测波长204 nm,柱温25℃。结果:通过对广西产柿叶进行薄层定性鉴别实验;并采用高效液相色谱法测定有效成分含量,齐墩果酸在0.230～2.30μg之间与峰面积呈良好的线性关系r,=0.9996,平均加样回收率为99.30%,RSD=1.8%(n=6);熊果酸在0.430～4.30μg之间与峰面积呈良好的线性关系r,=0.9999,平均加样回收率为99.68%,RSD=2.1%(n=6)。结论:实验中多批次测定结果表明,所建立的方法可用于柿叶薄层鉴别和齐墩果酸、熊果酸的含量测定。
Objective: To analyze the quality of persimmon leaves produced in Guangxi, in order to further improve the quality standards of persimmon leaves and provide the scientific basis for the qualitative and quantitative determination of persimmon leaves. Methods: The persimmon leaves in Guangxi were qualitatively identified by TLC. The contents of oleanolic acid and ursolic acid in persimmon leaves were determined by high performance liquid chromatography. The column was Thermo C18 (4.6 mm × 250 mm 5, μm) ), The mobile phase was methanol-0.2% ammonium acetate solution (80:20), the flow rate was 0.8 mL / min, the detection wavelength was 204 nm and the column temperature was 25 ℃. Results: The qualitative identification test of persimmon leaves in Guangxi was carried out. The content of active ingredients was determined by HPLC. The linear relationship between oleanolic acid and peak area was 0.230 ~ 2.30μg, r = 0.9996 , The average recovery was 99.30%, RSD = 1.8% (n = 6). Ursolic acid had a good linear relationship with the peak area of 0.430-4.30μg r = 0.9999 with an average recovery of 99.68 %, RSD = 2.1% (n = 6). Conclusion: The results from multiple batches in the experiment show that the established method can be used for the identification of thin layer of persimmon leaf and the determination of oleanolic acid and ursolic acid.