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[目的]分析血清有机氯农药水平与雌激素代谢基因CYP1A1易感基因型对乳腺癌患病风险的联合作用。[方法]采用成组病例对照研究方法,序贯收集乳腺癌确诊病例共102名,健康社区女性对照154名。采用气相色谱-电子捕获的方法(GC-ECD)检测血清中β-HCH,p,p’-DDT,和p,p’-DDE水平。用限制性内切酶PCR法(RFLP-PCR)法检测CYP1A1基因型的m1突变型。采用logistic回归模型分析CYP1A1突变基因型对乳腺癌患病风险的相对危险度(OR),并计算交互作用系数γ。[结果]总病例对照中,CYP1A1 m1突变纯合型(v/v)的调整OR=3.26,95%C.I.为1.12￣9.53。携带CYP1A1 m1突变等位基因(wv+vv)与有机氯农药(p,p’-DDT,β-HCH)高暴露的交互作用系数γ分别为8.86和8.47;绝经前女性中,上述交互作用系数γ分别为2.94和1.42。[结论]DDT及HCH可能是乳腺癌的危险因素,雌激素代谢相关的酶在其中起到协同促癌作用。
[Objective] To analyze the combined effect of serum levels of organochlorine pesticides and CYP1A1 susceptibility genotypes on the risk of breast cancer. [Methods] A total of 102 confirmed cases of breast cancer and 154 healthy women were enrolled in this study. Serum levels of β-HCH, p, p’-DDT, and p, p’-DDE were determined by gas chromatography-electron capture (GC-ECD) The m1 mutant of CYP1A1 genotype was detected by restriction endonuclease PCR (RFLP-PCR). The logistic regression model was used to analyze the relative risk (OR) of CYP1A1 genotypes for the risk of breast cancer, and the interaction coefficient γ was calculated. [Results] The total case control, CYP1A1 m1 mutation homozygous (v / v) adjustment OR = 3.26,95% C.I. is 1.12 ~ 9.53. The interaction coefficients γ carrying high exposure to CYP1A1 m1 mutant alleles (wv + vv) and organochlorine pesticides (p, p’-DDT, β-HCH) were 8.86 and 8.47, respectively. In premenopausal women, the above-mentioned interaction coefficient γ are 2.94 and 1.42, respectively. [Conclusion] DDT and HCH may be risk factors of breast cancer, estrogen metabolism-related enzymes play a synergistic role in cancer promotion.