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目的 :建立一种快速准确的硫酸软骨素测定方法。方法 :利用含有间苯三酚的无机酸与样品中硫酸软骨素反应生成有色产物进行分光光度分析。结果 :10 0℃反应 30min在波长 5 5 8nm处测定硫酸软骨素 ,在 0 .10～ 1.0mg/ml范围内线性关系良好 (r =0 .9993) ,具有较好重现性。 结论 :此法操作简便、结果准确。
Objective: To establish a rapid and accurate method for the determination of chondroitin sulfate. Methods: Spectrophotometric analysis was performed using inorganic acid containing phloroglucinol and chondroitin sulfate in the sample to produce colored products. Results: Chondroitin sulfate was detected at 558 nm at 10 0 ℃ for 30 min. The linearity was good in the range of 0.1-10 mg / ml (r = 0.9993), with good reproducibility. Conclusion: This method is simple and accurate.