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目的:研究白及多糖(RBP)对大鼠深Ⅱ度烫伤创面愈合过程中脂多糖(LPS)及Toll样受体4(TLR4)含量的影响。方法:30只SD大鼠,随机均分为空白对照组、模型和RBP组。复制大鼠深Ⅱ度烫伤创面模型,每天观察烫伤大鼠创面,记录创面愈合时间并计算创面愈合率。于烫伤后第1、3、7、14、21、28天分别通过尾静脉采血收集大鼠静脉血。采用鲎试剂检测血浆中LPS含量;采用酶联免疫吸附法检测血清中相关致炎细胞因子肿瘤坏死因子-α(TNF-α)、白介素-6(IL-6)的水平;采用流式细胞术检测不同时相点大鼠外周血中TLR4阳性表达率。结果:与模型组比较,RBP组大鼠创面愈合时间显著缩短(P<0.01);用RBP 7 d后,大鼠血清中LPS、TNF-α、IL-6水平显著降低(P<0.05或P<0.01),大鼠外周血中TLR4阳性表达率显著降低(P<0.05或P<0.01)。结论:RBP可促进大鼠深Ⅱ度烫伤创面愈合,其作用机制可能与降低烫伤大鼠LPS、TLR4以及相关的致炎细胞因子的表达有关。
Objective: To investigate the effects of RBP on lipopolysaccharide (LPS) and Toll-like receptor 4 (TLR4) during deep second degree scald wound healing in rats. Methods: Thirty SD rats were randomly divided into blank control group, model and RBP group. The rat deep second degree scald wound model was duplicated. The wounds of scalded rats were observed daily and wound healing time was recorded and wound healing rate was calculated. On the 1st, 3rd, 7th, 14th, 21st and 28th days after scalding, venous blood was collected from the tail vein by blood sampling. The levels of LPS in plasma were detected by limulus reagent. The levels of TNF-α and IL-6 in sera were detected by enzyme-linked immunosorbent assay (ELISA) The TLR4 positive rate in peripheral blood of rats at different time points was detected. Results: Compared with the model group, the wound healing time of RBP group was significantly shorter (P <0.01). After 7-day RBP treatment, the levels of LPS, TNF-α and IL-6 were significantly decreased <0.01). The positive rate of TLR4 in peripheral blood of rats was significantly decreased (P <0.05 or P <0.01). CONCLUSION: RBP can promote deep second degree scald wound healing in rats. The mechanism may be related to reducing the expression of LPS, TLR4 and related inflammatory cytokines in burned rats.