３２Ｐ探针杂交是病原微生物基因诊断应用最广泛的方法之一。在建立了人巨细胞病毒ＰＣＲ、套式ＰＣＲ的基础上，我们用６１３ｂｐＰＣＲ外引物扩增产物制备了杂交探针，并与３００ｂｐ内引物扩增产物进行转膜杂交，可将单纯琼脂糖凝胶电泳１７ｎｇ的检测水平提高到０．５ｎｇ，稀释梯度相当于提高２个数量级。因此，利用ＰＣＲ结合３２Ｐ杂交就可以检测出不到１个病毒的基因拷贝（１００ａｇ），为临床人巨细胞病毒潜伏感染的诊断提供了一种快速准确的诊断方法 32P probe hybridization is one of the most widely used methods for genetic diagnosis of pathogenic microorganisms. Based on the establishment of human cytomegalovirus PCR and nested PCR, we prepared the hybridization probe by using 613bp PCR primers and hybridized with the 300bp primer. The detection level of electrophoresis 17ng increased to 0.5ng, the dilution gradient is equivalent to an increase of 2 orders of magnitude. Therefore, the use of PCR combined with 32P hybridization can detect less than 1 gene copies of the virus (100ag), providing a rapid and accurate diagnostic method for the clinical diagnosis of human cytomegalovirus latent infection