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目的：观察苦参碱对 K562细胞 hTERT- mRNA表达及端粒酶活性的影响作用 ,并明确两者的相关性。方法：以 0.1～ 2 mg/ml苦参碱处理 K562细胞 48h后， RT- PCR检测其 hTERT- mRNA表达，同时行 TRAP- PCR- ELISA端粒酶活性检测。结果 :K562细胞为一强端粒酶活性细胞株，在浓度分别为 0.1、 0 5、 1 0、 2 0 mg/ml苦参碱作用后， K562细胞 hTERT- mRNA表达明显受抑，同时伴有端粒酶活性下降，抑制率分别为 0 3％、 13 0％、 91 7％和 98 6％。结论：苦参碱可降低 K562细胞 hTERT- mRNA表达 ,同时伴端粒酶活性下降；端粒酶活性强度与 hTERT- mRNA表达有关。
Objective: To observe the effect of matrine on hTERT-mRNA expression and telomerase activity in K562 cells, and to clarify the correlation between them. Methods: K562 cells were treated with 0.1-2 mg/ml matrine for 48 h. RT-PCR was used to detect the expression of hTERT-mRNA, and TRAP-PCR-ELISA was used to detect telomerase activity. RESULTS: K562 cells were a strong telomerase-producing cell strain, and the expression of hTERT-mRNA was significantly inhibited in K562 cells after treated with matrine at concentrations of 0.1, 0.5, 1.0, and 2.0 mg/ml, respectively. At the same time, the activity of telomerase was decreased, and the inhibition rates were 03%, 130%, 917% and 986%, respectively. CONCLUSION: Matrine can reduce the expression of hTERT-mRNA in K562 cells and decrease the telomerase activity. The intensity of telomerase activity is related to the expression of hTERT-mRNA.