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阴、阳离子交换层析综合分子筛层析过程可从 Cohn’s FⅣ中回收纯化人血白蛋白,所得白蛋白溶液呈淡黄色,澄清透明;纯度为99.1%;单体含量为97.3%;OD~(1%)_403nm1cm=0.031; PKA为2.81IU/ml。每公斤FⅣ可回收白蛋白45 g;白蛋白试制品能耐受57℃ 50 h的热稳定性试验。
Purification of human albumin from Cohn’s FIV by anion-cation exchange chromatography. The resulting albumin solution was pale yellow with a clear and transparent purity of 99.1%. The monomer content was 97.3 %; OD ~ (1%) _ 403nm1cm = 0.031; PKA 2.81IU / ml. Per kg of FⅣ recyclable albumin 45 g; albumin prototype can withstand 57 ℃ 50 h thermal stability test.