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目的:建立复方甘草麻黄碱片中盐酸麻黄碱和甘草浸膏(以甘草酸计)的质量控制方法。方法:采用C18(150mm×4.6mm,5μm)色谱柱;流动相:0.1%磷酸-乙腈溶液;流速:1.0mL·min-1;检测波长:盐酸麻黄碱207nm,甘草酸250nm。结果:盐酸麻黄碱在1.0～101.2μg·mL-1质量浓度范围内与峰面积呈良好的线性关系Y=21973.3X+1421.3(r=0.9995),回收率为100.34%,RSD=0.95%(n=6)。甘草酸在14.2～1416.0μg·mL-1质量浓度范围内与峰面积呈良好的线性关系Y=5872.7X-43188.1(r=0.9999),回收率99.30%,RSD=0.88%(n=6)。结论:本方法简便、快速、准确,能有效控制复方甘草麻黄碱片的质量。
Objective: To establish a quality control method of compound licorice ephedrine hydrochloride tablets and licorice extract (with glycyrrhizin). Methods: C18 (150mm × 4.6mm, 5μm) column was used. The mobile phase consisted of 0.1% phosphoric acid-acetonitrile solution and the flow rate was 1.0mL · min -1. The detection wavelength was 207nm for ephedrine hydrochloride and 250nm for glycyrrhizinate. Results: The linear range of peak area of ephedrine hydrochloride in the range of 1.0 ~ 101.2μg · mL-1 was Y = 21973.3X + 1421.3 (r = 0.9995), the recovery was 100.34%, RSD was 0.95% = 6). Glycyrrhizic acid had a good linear relationship with the peak area in the range of 14.2-1416.0μg · mL-1 Y = 5872.7X-43188.1 (r = 0.9999). The recovery was 99.30% and the RSD was 0.88% (n = 6). Conclusion: The method is simple, rapid and accurate, and can effectively control the quality of compound licorice ephedrine tablets.