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目的了解猪HBV的感染情况,探讨猪作为乙型肝炎病毒感染动物模型的可行性。方法采用酶联免疫吸附试验(ELISA)法对血清中的HBV血清标志物进行检测,分别用S区和C区引物对血清和白细胞中HBV-DNA进行聚合酶链式反应(PCR)以检测HBV的感染情况。结果①234份血清标本进行了HBV血清标志物的检测,HbsAg、HB-sAb、HBcAb、HBeAg、HBeAb的阳性率分别为4.70%(11/234)、29.06%(68/234)、79.91%(187/234)、2.99%(7/234)、15.81%(37/234),血清标记物全阴性16.67%(39/234);②血清HBV-DNA的PCR扩增结果:S区引物扩增阳性率3.4%(8/234),C区引物扩增阳性率20%(47/234);③白细胞DNAPCR扩增,S区扩增阳性率10.9%(17/156),C区扩增阳性率51.3%(80/156);④白细胞中HBV-DNA的检出率高于血清HBV-DNA的检出率,二者差异有统计学意义(P﹤0.001);⑤HBV-DNA的C区检出率高于S区检出率,二者差异有统计学意义。结论①HBV在屠宰生猪中有较高的感染率;②白细胞中HBV-DNA的检出率高于血清HBV-DNA的检出率;③HBV-DNA的C区检测比S区检测更为敏感。
Objective To understand the status of swine HBV infection and to explore the feasibility of swine as an animal model of hepatitis B virus infection. Methods Serum HBV markers were detected by enzyme-linked immunosorbent assay (ELISA), and HBV DNA in serum and leukocyte were detected by polymerase chain reaction (PCR) Of the infection. Results (1) Serum samples from 234 serum samples were tested for serum HBV markers. The positive rates of HBsAg, HBsAb, HBcAb, HBeAg and HBeAb were 4.70% (11/234), 29.06% (68/234), 79.91% /234), 2.99% (7/234), 15.81% (37/234) and 16.67% (39/234), respectively. ② The results of PCR amplification of serum HBV-DNA: Rate was 3.4% (8/234), and the positive rate of primer amplification in C region was 20% (47/234). ③ The positive rate of leukocyte DNAPCR amplification and S-region amplification was 10.9% (17/156) 51.3% (80/156); ④ The detection rate of HBV-DNA in leukocytes was higher than that of serum HBV-DNA, the difference was statistically significant (P <0.001); Rate higher than the S district detection rate, the difference was statistically significant. Conclusions ①HBV has a higher infection rate in slaughtered pigs. ② The detection rate of HBV-DNA in leucocytes is higher than that of serum HBV-DNA. ③ The detection of C region of HBV-DNA is more sensitive than the detection of S-region.