论文部分内容阅读
目的探讨Chemically-ActivatedLuciferaseeXpression(CALUX)生物学方法作为检测二的一种方法在环境流行病学调查中的实用性。方法分别采用高分辨率气相色谱和质谱仪联机(HRGC-MS)法和CALUX方法测定同一来源的人体脂肪、血液和母乳中二毒性当量,观察两种不同方法测定结果间的相关性。结果两种方法测定脂肪组织和母乳中二毒性当量结果之间的相关系数分别为0.8675和0.9581,具有显著的正相关关系(P<0.05)。二毒性当量>20pg/gfat的血液样品,两种方法测定结果之间也具有密切的相关关系(P<0.05或<0.01)。结论CALUX生物学方法可适用于一般人群的脂肪组织和乳汁样品以及二类暴露人群的血液中二类污染物的筛查。
Objective To investigate the practicability of a Chemically-Activated Luciferase-Xpression (CALUX) bioassay as a detection method in environmental epidemiological investigation. Methods Two toxic equivalents of human fat, blood and breast milk from the same source were determined by HRGC-MS method and CALUX method, respectively. The correlation between two different methods was observed. Results The correlation coefficients between the two methods for determining the two toxic equivalents in adipose tissue and breast milk were 0.8675 and 0.9581, respectively, with a significant positive correlation (P <0.05). Two toxic equivalent> 20pg / gfat blood samples, the two methods also have a close relationship between the results (P <0.05 or <0.01). Conclusion The CALUX biological method can be applied to the screening of two types of pollutants in the blood of the general population, adipose tissue and milk samples as well as the second-class exposed persons.