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以甜樱桃(Prunus avium L.)黄红皮品种‘雷尼’为母本,红皮品种‘8-100’为父本杂交获得的90株F1代群体为试材,利用RAPD、ISSR和SSR分子标记进行遗传分析,构建了含8个连锁群共50个分子标记(30条RAPD、15条ISSR、5对SSR标记)的遗传图谱,全长634.67cM,标记间的平均距离12.69cM。用基于混合线性模型的QTLNetwork2.0软件分析其果皮红色性状的QTL以及基因与环境的互作,发现了两个加性效应QTL和1对上位性互作QTL分布在Chr1和Chr7染色体上,两个加性效应的遗传贡献率(H2)分别为32.28%和47.52%,1对上位效应的遗传贡献率(H2AA)为37.87%,加性和上位性的效应位点与环境互作均为0。研究结果表明樱桃果皮红色性状的遗传受两个加性效应QTL和1对上位性互作QTL的影响。
Ninety F1 F1 progenies derived from the cross of Prunus avium L. yellow-red variety ’Raney’ as the female parent and the red-type ’8-100’ as the male parent were used as experimental materials, and RAPD, ISSR and SSR The molecular markers were used for genetic analysis, and the genetic map of 50 molecular markers (30 RAPDs, 15 ISSRs and 5 pairs of SSR markers) with 8 linkage groups was constructed. The full length was 634.67cM and the average distance between markers was 12.69cM. Using QTLNetwork2.0 software based on a mixed linear model to analyze the QTLs of the pericarp red traits and the interaction between the genes and the environment, two QTLs for additive effect and one pair of epistatic QTLs were found on the Chr1 and Chr7 chromosomes. Two The genetic contribution rate (H2) of two additive effects was 32.28% and 47.52%, respectively. The genetic contribution (H2AA) of one pair of epistatic effects was 37.87%. The additive effect and epistasis effect locus were both 0 . The results showed that the inheritance of red traits in cherry peel was affected by two additive QTLs and one epistatic QTL.