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以猪胰脂肪酶(PPL)代替传统的有机金属作为催化剂,在单模微波辐照下利用左旋丙交酯(L-LA)的开环聚合制备壳聚糖-左旋聚乳酸(CS-g-PLLA)接枝共聚物.考察了反应温度及酶用量对接枝率的影响.以此为基础,利用DTG、XRD和3T3成纤维细胞培养对产物的物理性能及细胞相容性进行分析.结果显示,猪胰酶可有效催化接枝反应的进行.反应温度和酶用量对产物的接枝率有较大影响.在单模微波作用下,较低的反应温度(50℃)可获得具有较高接枝率(178.8%)的接枝产物.与纯壳聚糖相比,接枝产物的结晶度和热稳定性降低,说明PLLA的引入破坏了壳聚糖的高结晶性.产物具有良好的细胞相容性,可作为优良的组织工程支架材料.
Using chitosan-pancreatic lipase (PPL) instead of traditional organometallic as catalyst, the chitosan-levulino-polylactic acid (CS-g- PLLA) grafted copolymer.The effects of reaction temperature and amount of enzyme on the grafting rate were investigated.The physical and cytocompatibility of the product were analyzed by DTG, XRD and 3T3 fibroblast culture.Results Showed that porcine trypsin can effectively catalyze the grafting reaction.The reaction temperature and the amount of enzyme have a significant impact on the grafting rate of the product.Under the single-mode microwave, the lower reaction temperature (50 ℃) Graft product with high grafting rate (178.8%) .Compared with pure chitosan, the crystallinity and thermal stability of grafted product decreased, indicating that the introduction of PLLA destroyed the high crystallinity of chitosan.The product has good The cell compatibility, can be used as excellent tissue engineering scaffold material.