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目的 探讨化疗前食管癌组织中胸苷酸合成酶(thymidylate synthase,TS)转录水平及其对顺铂/5 氟脲嘧啶(DDP/5 Fu)疗效的预测价值。方法 20 例食管癌患者均接受2 个周期DDP/5 Fu化疗。DDP 15~20 mg/m2/d,iv d1 5;5 Fu 375~500 mg/m2/d,ivgtt 8 h,d1 5,休息4 周后,进行第二个周期化疗。以β肌动蛋白(βactin)作内参照,应用半定量RT PCR技术检测化疗前食管癌癌组织中TSmRNA水平,以TS/Βactin比值表示。实验数据经SPSS 10.0统计软件处理,化疗有效和无效组间TSmRNA水平间差异分析采用Wilcoxon秩和检验;双侧检验,α取0.05作为显著性检验的标准。结果 20例食管癌患者中,11例化疗有效者的TS/βactin mRNA水平中位值为3.90×10 1,而9 例化疗无效者的TS/βactin mRNA水平中位值为9.10×10 1,两组差异具有统计学意义(P<0.05)。结论 化疗前食管癌组织中TS mRNA水平对应用DDP/5 Fu治疗食管癌的疗效具有一定预测价值。
Objective To investigate the transcriptional level of thymidylate synthase (TS) in esophageal cancer tissues before chemotherapy and the predictive value of cisplatin / 5-fluorouracil (DDP / 5 Fu). Methods Twenty patients with esophageal cancer underwent two cycles of DDP / 5 Fu chemotherapy. DDP 15 ~ 20 mg / m2 / d, iv d1 5; 5 Fu 375 ~ 500 mg / m2 / d, ivgtt 8 h, d1 5, after a rest for the second cycle of chemotherapy. Using β actin as an internal reference, semi-quantitative RT-PCR was used to detect TSmRNA in esophageal cancer tissues before chemotherapy, expressed as TS / Βactin ratio. The experimental data were processed by SPSS 10.0 statistical software. The differences of TSmRNA level between the two groups were analyzed by Wilcoxon rank-sum test. The two-sided test, α was taken as the standard of significance test. Results The median TS / βactin mRNA level of 20 patients with esophageal cancer was 3.90 × 10 1, while the median TS / βactin mRNA level of 9 chemotherapy-ineffective individuals was 9.10 × 10 1 There was significant difference between groups (P <0.05). Conclusions TS mRNA level in esophageal carcinoma before chemotherapy may have predictive value for the treatment of esophageal cancer with DDP / 5 Fu.