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目的研究早孕期绒毛细胞长期培养及染色体制备方法,评价其在产前诊断中的可行性。方法回顾性分析2001-01-2005-12北京协和医院77例早孕期绒毛细胞培养和染色体制备用于产前细胞遗传学诊断的病例。结果平均培养时间为5～7d,一般10d可以发报告,77例病例中有1例培养失败,培养成功率98.7%,培养过程中细菌污染率为0,母体组织污染率为0。发现异常核型5例,胎盘限制性嵌合体2例。结论绒毛细胞长期培养法和染色体制备方法简单、技术稳定、结果可靠,可应用于产前诊断。
Objective To study the long-term culture of chorionic villi in early pregnancy and the method of chromosome preparation to evaluate its feasibility in prenatal diagnosis. Methods A retrospective analysis of 77 cases of early pregnancy chorionic villi cell culture and chromosome preparation for prenatal cytogenetic diagnosis was performed in 77 Peking Union Medical College Hospital from January 2001 to December 2005. Results The average culture time ranged from 5 days to 7 days. It could be reported after 10 days. One out of 77 cases failed to cultivate. The success rate of cultivation was 98.7%. The bacterial contamination rate was 0 and the rate of maternal tissue contamination was 0. Abnormal karyotypes were found in 5 cases and placenta-restricted chimeras in 2 cases. Conclusion Long-term culture of villus cells and chromosome preparation method is simple, stable technology, reliable results, can be applied to prenatal diagnosis.