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目的考察氯胺酮对大鼠肝微粒体内细胞色素P450_2B酶活性的影响。方法以10mg.kg-1.d-1氯胺酮灌胃给予大鼠,连续给药10d。在第11d处死大鼠,以钙离子沉淀法制备肝微粒体。采用Omura和Sato方法测定细胞色素P450含量,采用7-戊氧基试卤灵-O-去烷基反应测定细胞色素P450_2B酶的活性。结果给药组和对照组细胞色素P450的总含量分别是(0.679±0.216)nmol·mg-1和(0.398±0.109)nmol·mg-1,细胞色素P450_2B酶的活性分别是(13.40±3.15)pmol·min-1.mg-1和(7.04±2.09)pmol·min-1.mg-1。结论氯胺酮能够增加大鼠肝微粒中细胞色素P450含量,并能显著诱导细胞色素P450_2B酶的活性。
Objective To investigate the effect of ketamine on cytochrome P450 2 B activity in rat liver microsomes. Methods Rats were given gavage with 10 mg.kg-1.d-1 ketamine for 10 days. Rats were sacrificed on day 11 and liver microsomes were prepared by calcium ion precipitation. The content of cytochrome P450 was measured by Omura and Sato methods. The activity of cytochrome P450 2 B enzyme was determined by 7-pentoxy-resorufin-O-dealkylation reaction. Results The total contents of cytochrome P450 in the treated group and the control group were (0.679 ± 0.216) nmol · mg-1 and (0.398 ± 0.109) nmol · mg-1, respectively. The activities of cytochrome P450_2B were (13.40 ± 3.15) pmol · min-1.mg-1 and (7.04 ± 2.09) pmol · min-1.mg-1. Conclusion Ketamine can increase the content of cytochrome P450 in rat liver particles and induce the cytochrome P450 2 B enzyme activity remarkably.