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目的 探讨慢性间质性肾炎发生发展中炎症细胞浸润与粘附因子表达的特点。方法 用雌性Ｗｉｓｔｅｒ 大鼠，皮下注射嘌呤霉素氨基核苷（ＰＡＮ） 建立慢性间质性肾炎动物模型。于注射后０ 、３ 、５ 、８、１２ 周分别检测２４ 小时尿蛋白定量，分批取出肾脏作ＨＥ染色，免疫组织化学ＡＢＣ法标记单克隆抗体ＣＤ４、ＣＤ８、ＭΦ、粘附分子１（ＩＣＡＭ１） 、ＣＤ１１ａ、ＣＤ１１ｂ、ＣＤ１８、ＣＤ４４ 、ＨＡ、ＴＣＲ（α，β）、ＭＨＣⅡ。结果 实验组第３ 周始２４ 小时尿蛋白量逐渐增加，与对照组比较有显著性差异，ＨＥ染色可见肾间质炎症细胞浸润，ＣＤ４ 阳性、ＴＣＲ阳性、ＩＣＡＭ１ 阳性细胞增多，第８ 周２４ 小时尿蛋白量达高峰，出现肾间质纤维化，肾小管萎缩、ＩＣＡＭ１、ＴＣＲ、ＣＤ４４、ＨＡ、ＣＤ１１ａ、ＣＤ１１ｂ、ＣＤ１８、ＭＨＣ Ⅱ阳性细胞数达高峰；第１２ 周，多种细胞因子表达及２４ 小时尿蛋白定量呈下降趋势。结论 ＣＤ４ 阳性细胞的浸润增殖，启动了多种细胞因子表达，在慢性间质肾炎发生发展中起重要作用。
Objective To investigate the characteristics of inflammatory cell infiltration and adhesion factor in the development of chronic interstitial nephritis. Methods Female Wister rats were injected subcutaneously with puromycin aminonucleoside (PAN) to establish an animal model of chronic interstitial nephritis. Urine protein was measured 24 hours after injection at 0, 3, 5, 8 and 12 weeks after injection. Kidneys were removed for HE staining. Immunohistochemical ABC method was used to label monoclonal antibodies CD4, CD8, MΦ, adhesion molecule 1 1), CD11a, CD11b, CD18, CD44, HA, TCR (α, β), MHC Ⅱ. Results In the experimental group, the amount of urinary protein gradually increased 24 hours after the third week, which was significantly different from the control group. HE staining showed infiltration of interstitial inflammatory cells, CD4 positive, TCR positive, ICAM 1 positive cells increased, 24 hours urinary protein peak, renal interstitial fibrosis, tubular atrophy, ICAM 1, TCR, CD44, HA, CD11a, CD11b, CD18, MHC Ⅱ-positive cells reached the peak; the first 12 weeks, a variety of cells Factor expression and 24-hour urine protein decreased. Conclusion The infiltration and proliferation of CD4-positive cells initiate a variety of cytokine expression and play an important role in the development of chronic interstitial nephritis.