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在以猪粪为底物的沼气发酵系统中,投加产氢菌Clostridium sp.WY-1与Clostridium sp.WW-5混合后离心的菌体,加入量以两株菌的混合菌液计,分别为发酵液总体积的2%、4%、8%、16%,30℃下发酵30 d,研究产氢菌投加对产甲烷系统的生物强化效应.结果表明,2%、4%、8%、16%四种不同投加量均能提高甲烷总产量,累积甲烷产量比对照分别增加6%、11%、4%、3%,其中投加量4%增加最高;而对甲烷浓度无明显提高.在发酵前3 d,各组中均能检测到少量的氢气,投加产氢菌各组中d 2的氢气含量均比对照高,d 3对照组、2%和4%组检测不出氢气,但8%、16%组仍有极少量氢气存在.总挥发性有机酸在发酵过程中总体趋势是不断下降,发酵前20 d投加产氢菌组的总挥发性有机酸含量均比对照组高.投加产氢菌各组中产氢菌和产甲烷菌的数量与对照组相比均有所增加,而投加量4%组中产氢菌和产甲烷菌的数量比投加2%、8%和16%中的产氢菌和产甲烷菌的数量高.
In the pig manure biogas fermentation system, adding hydrogen production bacteria Clostridium sp. WY-1 and Clostridium sp. WW-5 mixed after centrifugation of bacteria, the amount of bacteria in two strains of bacteria, The biofortification effects of hydrogen production bacteria on the methanogenic system were studied by fermentation at 2%, 4%, 8%, 16% and 30 ℃ for 30 days respectively.The results showed that 2%, 4% 8%, 16% of the four different dosage can increase the total methane production, cumulative methane production increased by 6%, 11%, 4%, 3%, respectively, of which the highest increase of 4%; methane concentration No significant increase in the first 3 days before fermentation, each group can detect a small amount of hydrogen, hydrogen production bacteria d 2 hydrogen production in each group were higher than the control, d 3 control group, 2% and 4% group No hydrogen was detected, but there was still a small amount of hydrogen in the 8% and 16% groups.The overall trend of the total volatile organic acids in the fermentation process continued to decline, and the total volatile organic acids Were higher than those in the control group.The numbers of hydrogen-producing bacteria and methanogenic bacteria in each group were increased compared with the control group, but the number of hydrogen-producing bacteria and methanogenic bacteria in the 4% Add 2% , 8% and 16% of the number of hydrogen producing bacteria and methanogenic bacteria.