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研究去大脑皮层血管对大鼠侧脑室室管膜下增殖分化为新的神经元和胶质细胞的作用和机制。以去大鼠脑皮层血管为模型,以Nestin,BrdU,Tuj1,vimentin,bFGF为指标,采用免疫组织化学和图象分析方法,研究去大脑皮层血管对大鼠侧脑室室管膜下增殖分化为新的神经元的作用和机制。去皮层血管后大鼠侧脑室各水平SVZ的Nestin,BrdU,Tuj1,vimentin,bFGF阳性细胞的数目明显增多,形态显著改变。模型组15天、30天侧脑室前角外侧壁SVZ的Nestin,BrdU,Tuj1,vimentin阳性细胞计数均多于正常组,差异性显著(P<0.01),具有统计学意义。模型组15天和30天前角外侧壁SVZ的bFGF阳性细胞的面密度和光密度均高于正常组,有显著性差异(P<0.01),具有统计学意义。去皮层损伤刺激可引起脑内bFGF水平上调,促进SVZ神经干细胞增殖,并分化新的神经元和胶质细胞,可能参与大脑损伤修复。
To investigate the effects and mechanisms of cerebral cortical blood vessels on the proliferation and differentiation of rat lateral ventricle into new neurons and glial cells. Using the rat cerebral cortical blood vessels as a model, using Nestin, BrdU, Tuj1, vimentin, bFGF as an indicator, using immunohistochemistry and image analysis methods to study the cerebral cortex vascular proliferation and differentiation of rat lateral ventricle into The role and mechanism of new neurons. The numbers of Nestin, BrdU, Tuj1, vimentin and bFGF positive cells in SVZ at each level of lateral cerebral ventricle were significantly increased after the cortical vessels were removed, and the morphology was significantly changed. The positive rate of Nestin, BrdU, Tuj1 and vimentin in the SVZ of lateral ventricle on the 15th and 30th day in model group were all significantly higher than those in normal group (P <0.01), which were statistically significant. The surface density and optical density of bFGF-positive cells in SVZ of model group were higher than those of normal group at 15 days and 30 days (P <0.01), which was statistically significant. Cortical damage stimulus can cause brain bFGF levels, promote SVZ neural stem cell proliferation and differentiation of new neurons and glial cells, may participate in brain injury repair.