论文部分内容阅读
用Ames试验、噬菌体诱导试验、SOS固体显色试验3种测试方法对临床使用的22种抗肿瘤药物进行了致突变性检测.以临床用药浓度的基础将各种检品稀释25～625倍,以稀释剂蒸馏水、二甲基亚砜为阴性对照剂,以四硝基喹啉-N-氧化物、2-氨基芴和丝裂霉素为阳性对照剂.各种试验方法均做加与不加大鼠肝脏微粒体酶活化系统的2种试验.结果发现:22种抗肿瘤药物中有15种致突变阳性,最低致突变浓度炎1/625;原噬菌体诱导试验的阳性率为14/20,Ames试验的阳性率为10/21,SOS固
Ames test, phage induction test, SOS solid color test three kinds of test methods for clinical use of 22 kinds of anti-tumor drugs were mutagenicity test.According to the concentration of clinical medication will be a variety of test items diluted 25 to 625 times, Diluent distilled water, dimethyl sulfoxide as negative control, with four nitroquinoline-N-oxide, 2-aminofluorene and mitomycin as positive control agent.All the test methods are plus and not Two kinds of experiments of increasing rat liver microsomal enzyme activation system.The results showed that: 15 kinds of 22 kinds of antitumor drugs were mutagenic positive, the lowest mutagenic concentration of inflammation 1/625; prophage phage induction test was 14/20 Ames test positive rate of 10/21, SOS solid