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美国山核桃是扦插后极难生根的树种,为研究其不定根生根机制,利用MALDI-TOF-MS技术对美国山核桃不定根发育关键时期的差异蛋白进行鉴定分析,发现了48个表达相对差异的蛋白点,选择其中18个差异蛋白进行肽质谱指纹分析并进行同源性比较。结果表明,美国山核桃不定根形成的生理过程中产生了差异蛋白质,其中包括了能量代谢相关蛋白、逆境胁迫相关蛋白和信号传递相关蛋白等差异蛋白。分析可知:能量代谢相关蛋白有ATP合成相关酶类、光合作用相关蛋白及烯醇化酶等;逆境胁迫相关蛋白与热激蛋白同源;信号传递相关蛋白主要为细胞色素酶类和血红结合蛋白。
In order to study the rooting mechanism of adventitious rooting, MALDI-TOF-MS was used to identify and analyze the differential proteins in adventitious root development of Hickory in USA. 48 proteins with relative difference were found The 18 differential proteins were selected for peptide mass fingerprinting analysis and homology comparison. The results showed that differential proteins were produced in the physiological processes of adventitious root formation in American hickory, which included energy metabolism related proteins, stress response related proteins and signal transduction related proteins. Analysis shows that: energy metabolism-related proteins ATP-related enzymes, photosynthesis-related proteins and enolase; stress-stress-related proteins and heat shock protein homology; signal transduction-related proteins are mainly cytochrome enzymes and hemoglobin .