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本试验将禾谷镰孢一高产毒菌株 B4 - 1采用单小花注滴法接种于小麦抗赤霉病品种苏麦 3号和感病品种宁麦 6号 ,用气 /质联检法测定接种后 4、8、12、16和 2 0 d组织中的单端孢霉烯族毒素积累量。结果显示 ,在病组织中有脱氧雪腐镰刀菌烯醇 (deoxynivalenol,DON)、15 -乙酰脱氧雪腐镰刀菌烯醇(15 - acetyldeoxynivalenol,15 - ADON )和 3-乙酰脱氧雪腐镰刀菌烯醇 (3- acetyldeoxynivalenol,3-ADON)等 3种单端孢霉烯族毒素 ,但未发现雪腐镰刀菌烯醇 (nivalenol)和镰刀菌酮 - X(fusarenon- X)等毒素。抗病品种病穗组织中 ,DON平均含量由接种后 4 d的 2 9.5 μg/ g dry weight(gdw)下降到 2 0d时的 15 .7μg/ gdw,15 - ADON和 3- ADON量则较低 ,分别为 1.5～ 3.6μg/ gdw和 <1.7μg/ gdw;而同期感病品种病穗组织中的 DON量则由 2 3.1μg/ gdw上升到 4 7.7μg/ gdw,15 - ADON和 3- ADON量均较高 ,分别达 8.6～ 10 .0μg/ gdw和 2 .6～ 8.3μg/ gdw。在抗、感病品种无症状穗组织中 ,3种毒素的含量均很低 (<0 .5 μg/ gdw)。同时 ,本研究还首次在感病品种的穗颈 (总花梗 ,peduncle)组织中检测到大量的 DON和 15 - ADON及少量的 3- ADON。
In this experiment, a high yielding strain of Fusarium graminearum, B4 - 1, was inoculated on the wheat scab variety Sumai 3 and the susceptible cultivar Ningmai 6 by using a single flower injection method. After the inoculation Trichothecenes Toxins Accumulation in Tissues at 4, 8, 12, 16 and 20 days. The results showed that deoxynivalenol (DON), 15 - acetyldeoxynivalenol (15 - ADON) and 3 - acetyl deoxynivalenol 3 kinds of trichothecenes such as 3-acetyldeoxynivalenol (3-ADON), but no toxins such as nivalenol and fusarenon-X were found. The average content of DON decreased from 2 9.5 μg / g dry weight (gdw) at 4 days after inoculation to 15.7 μg / gdw at 20 days, while the contents of 15 - ADON and 3 - ADON were lower , Which were 1.5-3.6μg / gdw and <1.7μg / gdw, respectively. However, the amount of DON in spike tissues of susceptible varieties increased from 21.1μg / gdw to 4 7.7μg / gdw, 15 - ADON and 3 - ADON The results were as high as 8.6-10.0 μg / gdw and 2.6-6.3 μg / gdw, respectively. The content of all three toxins was very low (<0.5 μg / gdw) in the asymptomatic panicle of resistant and susceptible cultivars. In the meantime, a large amount of DON, 15 - ADON and a small amount of 3 - ADON were also detected in the panicle (peduncle) tissues of susceptible cultivars for the first time.