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利用文心兰的茎尖生长锥诱导出原球茎后，通过症状观察、生物接种、电镜观察和血清学检测方法进行了ＣＭＶ、ＴＭＶ、ＣｙＭＶ、ＯＲＳＶ等４种常见兰花病毒病的检测，成功地建立了３个品种的文心兰脱病毒无性系，采用经改良的ＫＣ培养基和１／２ＭＳ培养基有利于文心兰原球茎的快速繁殖，添加０．２ｍｇ／ＬＮＡＡ和１ｍｇ／Ｌ６－ＢＡ的培养基对文心兰原球茎增殖效果较好。ＭＳ培养基中添加１ｍｇ／ＬＩＡＡ有利于文心兰脱毒苗的生根。
After the protocorm-like bodies were induced by the cone tips of Oncidium, CMV, TMV, CyMV and ORSV were detected by symptom observation, biological inoculation, electron microscopy and serological test. The establishment of three species of avenaceae detoxification clones, the use of improved KC medium and 1 / 2MS medium is conducive to the rapid propagation of centropubic bulb, add 0.2mg / LNAA and 1mg / L6-BA The culture medium has good effect on the proliferation of the genus Ventura. MS medium supplemented with 1mg / L IAA is conducive to the rooting of Atraxin seedling.