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目的通过金黄色葡萄球菌的双溶血环菌落特征及药物敏感性检测,为临床快速诊断和治疗提供依据。方法对2014年8月-2015年5月从患者送检的不同标本中分离出19株双溶血环菌株进行革兰染色镜检及血浆凝固酶试验,利用全自动微生物分析仪进行菌种鉴定和药敏试验,并对其中1株进行了16SrRNA基因序列比对分析,同时对16种常用抗菌药物进行药敏试验分析。结果双溶血环金黄色葡萄球菌菌落直径较大,内环为狭窄的β溶血,外环为宽大的α溶血,血浆凝固酶结果均呈阳性,菌种鉴定均为金黄色葡萄球菌;其中共分离出6株MRSA,检出率占31.6%;19株双溶血环金黄色葡萄球菌体外药敏试验结果显示,万古霉素、利奈唑胺、喹奴普汀/达福普汀、呋喃妥因及替加环素对金黄色葡萄球菌抗菌作用最强,敏感率均为100.0%,对利福平、莫西沙星、庆大霉素、四环素、磺胺甲噁唑/甲氧苄啶等也表现出很好的敏感性,对青霉素的耐药率最高为84.2%。结论根据金黄色葡萄球菌所具有的双溶血环的特殊菌落特征和生化试验,能够快速准确鉴定,并根据药敏试验结果合理用药,有效控制金黄色葡萄球菌的感染,以减少耐药菌株的产生和流行。
Objective To detect the double hemolytic colony of Staphylococcus aureus and provide a basis for rapid clinical diagnosis and treatment. Methods 19 strains of hemolytic bacteria isolated from different samples were collected from patients in August 2014-May 2015 for Gram stain microscopy and coagulase test. The strains were identified by automatic microbiological analyzer Antimicrobial susceptibility test, and one of them was 16SrRNA gene sequence alignment analysis, at the same time, 16 commonly used antimicrobial drugs susceptibility test analysis. Results Staphylococcus aureus colonies were larger in diameter, with narrow β-hemolysis in the inner ring and wide α-hemolysis in the outer ring. Staphylococcus aureus was positive for the coagulase, Six strains of MRSA were detected with a detection rate of 31.6%. In vitro susceptibility test of 19 strains of Staphylococcus aureus showed that vancomycin, linezolid, quetiapine / dalfopristin, nitrofurantoin and teglutinin Cyclosporine has the strongest antibacterial effect on Staphylococcus aureus, the sensitivity rates are 100.0%, and it also shows good effects on rifampicin, moxifloxacin, gentamicin, tetracycline, sulfamethoxazole / trimethoprim The sensitivity of penicillin was 84.2%. Conclusions According to the special colony characteristics and biochemical tests of double hemolytic ring in Staphylococcus aureus, it can be quickly and accurately identified, and according to the drug susceptibility test results, it can be used rationally to effectively control the infection of Staphylococcus aureus to reduce the production of resistant strains And popular.