将人酸性纤维母细胞生长因子’（ａＦＧＦ’）ｃＤＮＡ的ＰＣＲ产物以ＴＡ连接方式克隆入ｐＣＲ￣（ＴＭ）ＩＩ质粒，然后采用Ｔ７和Ｓｐ６启动子特异性引物对克隆的片段以双脱氧未端终止法进行双链ＤＮＡ测序。结果表明这项技术是一种快捷而可靠的克隆及分析ＰＣＲ产物的方法 The PCR product of the human sFGF cDNA was cloned into the pCR ™ (TM) II plasmid by TA ligation and the cloned fragment was digested with the T7 and Sp6 promoter-specific primers Termination method for double-stranded DNA sequencing. The results show that this technique is a fast and reliable method for cloning and analyzing PCR products