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目的探讨多效生长因子(pleiotrophin,PTN)mRNA及其蛋白在结直肠癌组织中的表达及其临床意义。方法采用原位杂交和免疫组化染色方法,检测结直肠癌组织和正常结直肠组织中PTN mRNA及其蛋白的表达,并分析其与结直肠癌患者临床病理特征的关系。结果结直肠癌组PTN mRNA及其蛋白的表达阳性率分别为63.9%(53/83)和60.2%(50/83),均高于(P=0.008,P=0.002)正常对照组的40.7%(22/54)和33.3%(18/54)。结直肠癌组织中PTN mRNA及其蛋白的表达与肿瘤分化程度和TNM分期均有关(P<0.05),Ⅲ～Ⅳ期者和低分化者的表达阳性率均较高;而与年龄、性别及肿瘤类型均无关(P>0.05)。结论 PTN mRNA及其蛋白在结直肠癌组织中高表达,其可能参与了结直肠癌的浸润和转移。PTN可作为预测结直肠癌转移和预后的指标之一。
Objective To investigate the expression of pleiotrophin (PTN) mRNA and its protein in colorectal cancer and its clinical significance. Methods The expression of PTN mRNA and its protein in colorectal cancer tissues and normal colorectal tissues was detected by in situ hybridization and immunohistochemical staining. The relationship between the expression of PTN mRNA and the clinicopathological characteristics of colorectal cancer was analyzed. Results The positive rates of PTN mRNA and protein in colorectal cancer were 63.9% (53/83) and 60.2% (50/83), respectively, which were higher than those in the control group (P = 0.008, P = 0.002) (22/54) and 33.3% (18/54). The expression of PTN mRNA and its protein in colorectal cancer tissues was correlated with tumor differentiation and TNM stage (P <0.05), while those in stage Ⅲ ~ Ⅳ and poorly differentiated were higher Tumor types had no correlation (P> 0.05). Conclusion PTN mRNA and its protein are highly expressed in colorectal cancer tissues, which may be involved in the invasion and metastasis of colorectal cancer. PTN can be used as one of the indicators to predict the metastasis and prognosis of colorectal cancer.