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对本院用于抗生育的自制制剂黄芫花乙醇液进行了毒性及质量标准研究。测得其ＬＤ５０为２．１６ｇ／ｋｇ。以河朔荛花素为对照品，苯乙酸乙酯（１∶０．５）为展开剂，在ＧＦ２５４薄层板上分离，定性了黄芫花乙醇液。以ＨＰＬＣ，采用外标法测定黄芫花乙醇液中河朔荛花素的含量；甲醇—水（８０∶２０）为流动相，ＵＶ检测波长为２５４ｎｍ，河朔荛花素在１．０２～５．１０μｇ范围内与峰面积线性关系良好（ｒ＝０．９９９８）。加样回收率为９９．１３％，相对标准偏差为０．６３％（ｎ＝９）。
Toxicity and quality standards were studied on the self-made anti-fertility Astragalus ethanol solution used in this hospital. Its LD50 was measured to be 2.16 g/kg. With geranine as the reference substance, benzene-ethyl acetate (1:0.5) as the developing agent, it was separated on the GF254 thin-layer plate, and the ethanol solution of radix scutellariae was identified. HPLC method was used to determine the content of cortidine in the ethanol solution of Scutellaria baicalensis Georgi with external standard method; methanol-water (80:20) was used as the mobile phase, UV detection wavelength was 254 nm, cortidine was 1.02-5. The linear relationship with peak area was good in the range of 10 μg (r = 0.9998). The recovery was 99.13% with a relative standard deviation of 0.63% (n=9).