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目的优化盐酸肾上腺素诱导建立斑马鱼血栓模型的条件,并考察此模型在中药抗血栓活性筛选中的适用性。方法应用正交实验,以斑马鱼尾静脉红细胞的染色强度为定量指标,考察盐酸肾上腺素诱导建立斑马鱼血栓模型的最佳条件。利用该模型,对临床常用的11味中药的抗血栓活性进行筛选,探讨该模型在中药抗血栓活性筛选中的适用性与可靠性。结果盐酸肾上腺素诱导建立斑马鱼血栓模型的最佳条件为:受精后3天(3 dayspost fertilization,3dpf)的斑马鱼在浓度为15μmol/L盐酸肾上腺素溶液中暴露16小时。9味活血化瘀中药川芎、郁金、延胡索、丹参、红花、虎杖、三棱、莪术、水蛭在斑马鱼血栓模型中表现出显著的预防和治疗血栓作用,而甘草和党参两味中药在斑马鱼血栓模型中未表现出预防和治疗血栓的作用。结论盐酸肾上腺素诱导建立的斑马鱼血栓模型在中药抗血栓活性的筛选与疗效评估中具有良好的适用性。
Objective To optimize the conditions for the establishment of zebrafish thrombus induced by epinephrine hydrochloride and investigate the applicability of this model in the screening of antithrombotic activity of traditional Chinese medicine. Methods Using orthogonal experiment, the staining intensity of erythrocytes in zebrafish tail veins was used as a quantitative index to investigate the optimal conditions of zebrafish thrombosis induced by epinephrine hydrochloride. Using this model, the antithrombotic activity of 11 herbs commonly used in clinical trials was screened to explore the applicability and reliability of this model in the screening of antithrombotic activity of traditional Chinese medicine. Results The optimal conditions of zebrafish thrombosis induced by epinephrine hydrochloride were as follows: zebrafish exposed to 15 μmol / L epinephrine hydrochloride at 3 days post fertilization (3 days post fertilization, 3dpf) for 16 hours. Rhizoma Chuanxiong, tulip, corydalis root, Salvia miltiorrhiza, safflower, Polygonum cuspidatum, Prunus armeniaca, Curcuma longa and Leech showed significant prevention and treatment of thrombus in zebrafish thrombus model. The zebrafish thrombus model did not show the effect of preventing and treating thrombus. Conclusion The model of zebrafish thrombosis induced by epinephrine hydrochloride has good applicability in the screening and curative effect evaluation of antithrombotic activity of traditional Chinese medicine.