论文部分内容阅读
目的:比较正常胃黏膜、残胃炎、胃癌、残胃癌中hMLH1、hMSH2、c-Myc及PTEN蛋白表达情况,探讨上述蛋白在残胃癌发生、发展中的作用。方法:用免疫组化SP法检测hMLH1、hMSH2、c-Myc、PTEN蛋白在20例正常胃黏膜、20例残胃炎、38例胃癌、18例残胃癌组织中hMLH1、hMSH2、c-Myc、PTEN蛋白的表达情况。结果:在残胃癌中hMLH1、hMSH2蛋白的阳性率分别为50.0%,44.4%,分别低于正常胃黏膜组织100.0%,100.0%(P<0.01)。胃癌中hMLH1、hMSH2的表达阳性率为68.4%,63.2%,与残胃癌相比无显著差异(P>0.05)。c-Myc蛋白在残胃炎黏膜组织的表达阳性率(45.0%)要高于正常胃黏膜(0.0%)(P<0.05),而残胃癌中的表达阳性率(61.1%)与胃癌中的表达(55.3%)差异无显著性(P>0.05)。PTEN蛋白在残胃癌(44.4%)和胃癌(47.4%)中的表达均下调,两组之间差异无统计学意义(P>0.05)。结论:残胃癌的发生可能与错配修复系统失活有关,癌基因c-myc激活和抑癌基因PTEN的失活也参与了残胃癌的发生、发展。
OBJECTIVE: To compare the expression of hMLH1, hMSH2, c-Myc and PTEN in normal gastric mucosa, residual gastritis, gastric cancer and gastric residual gastric cancer, and to explore the role of these proteins in the occurrence and development of gastric residual gastric cancer. Methods: The expressions of hMLH1, hMSH2, c-Myc and PTEN protein in 20 cases of normal gastric mucosa, 20 cases of residual gastritis, 38 cases of gastric cancer and 18 cases of residual gastric cancer were detected by immunohistochemical SP method. Protein expression. Results: The positive rates of hMLH1 and hMSH2 protein in gastric stump were 50.0% and 44.4%, respectively, which were lower than those in normal gastric mucosa (100.0% and 100.0%, P <0.01). The positive rates of hMLH1 and hMSH2 in gastric cancer were 68.4% and 63.2%, respectively, which were not significantly different from those in gastric cancer (P> 0.05). The positive expression rate of c-Myc in gastric mucosa (45.0%) was higher than that in normal gastric mucosa (0.0%) (P <0.05), while the expression of c-Myc in gastric mucosa (55.3%) was no significant difference (P> 0.05). PTEN protein expression was down-regulated in gastric cancinoma (44.4%) and gastric cancer (47.4%), with no significant difference between the two groups (P> 0.05). Conclusion: The occurrence of gastric residual gastric cancer may be related to the inactivation of mismatch repair system. The activation of c-myc oncogene and the inactivation of tumor suppressor gene PTEN are also involved in the occurrence and development of gastric residual gastric cancer.