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本文用Sephadex G-200凝胶过滤法将猪眼水溶性晶体蛋白质分成α—、β_H~-、β_L~-和γ—晶体蛋白。它们在含有SDS—的10%聚丙烯酰胺凝胶电泳图谱中,显出不同电泳区带,α—晶体蛋白显示二条区带,β_H~-晶体蛋白显示六条区带,β_L~-晶体蛋白显示三条区带,γ—晶体蛋白呈现二条区带。用7.6M 尿素溶液处理水不溶性晶体蛋白质,将其分成尿素溶性晶体蛋白质和尿素不溶性晶体蛋白质两部分;以含SDS 的10%聚丙烯酰胺凝胶电泳测定,尿素溶性晶体蛋白质的主要成分的分子量是20,000和43,000,而尿素不溶性晶体蛋白质的主要成分的分子量约为20,000。
In this paper, Sephadex G-200 gel filtration method is used to separate porcine water-soluble crystal proteins into α-, β_H ~ -, β_L ~ - and γ-crystal proteins. They showed two bands in the electrophoresis band of 10% polyacrylamide gel electrophoresis with SDS-PAGE. The α-crystallin showed two bands and β_H ~ - crystal protein showed six bands. The β_L ~ - crystal protein showed three bands Zone, γ-crystal protein showed two bands. Water insoluble crystal protein was treated with 7.6M urea solution, which was divided into two parts, urea-soluble crystal protein and urea-insoluble crystal protein. The molecular weight of the main component of urea-soluble crystal protein was 10% polyacrylamide gel electrophoresis with SDS 20,000 and 43,000, while the major component of the urea insoluble crystalline protein has a molecular weight of about 20,000.