本文介绍一种用增大血清标本用量,以提高检出灵敏度的甲胎蛋白(AFP)“井形”免疫电泳测定法。本法操作方便,设备简单,对AFP的检出灵敏度可达20～30ng/ml左右。 一、材料 (一)井型模板(图1):取有机玻璃3×80×100mm一块,10×15×94mm一块(以等距钻打直径6mm孔),2×3×10mm二条,用三氯甲烷按图1粘合而成。 This article describes an AFP well pattern immuno-electrophoresis assay that increases the amount of serum used to increase the sensitivity of detection. This method is easy to operate, the device is simple, the detection sensitivity of AFP up to 20 ~ 30ng / ml or so. First, the material (a) well-type template (Figure 1): Take a piece of plexiglass 3 × 80 × 100mm, a 10 × 15 × 94mm (drilling 6mm diameter holes at equal intervals), 2 × 3 × 10mm two, Chloromethane according to Figure 1 bonded together.